We are excited to share our latest study, in which we investigated how dynamic protein networks shape tissue formation during vertebrate development in collaboration with the Vermeulen lab.
We adapted our previously published microfluidic system to synchronize oscillations in cultured mouse embryo tails and combined this with proteomics and RNA sequencing. This approach allowed us to map spatiotemporal protein and gene expression patterns during somitogenesis.
With this dataset, we identified previously unknown oscillatory proteins and discovered a dynamic, antagonistic ligand–receptor pattern in R-Spondin/LGR signalling. This mechanism explains how Wnt-oscillation amplitude increases despite decreasing ligand levels in the anterior presomitic mesoderm.
For more details, read the full preprint here.
