We are pleased to announce the publication of a study to which we contributed, “Deciphering lineage specification during early embryogenesis in mouse gastruloids using multilayered proteomics”, in Cell Stem Cell. This research represents a significant advancement in understanding the molecular underpinnings of early mammalian development.
Under the leadership of Suzan Stelloo from the lab of Michiel Vermeulen, we employed a comprehensive mass spectrometry-based proteomics approach to investigate the dynamics of protein expression during gastruloid differentiation. Our findings revealed distinct temporal expression patterns and unique profiles for each germ layer, validated through single-cell proteomics technology. Additionally, we profiled enhancer interaction landscapes using P300 proximity labeling, identifying numerous gastruloid-specific transcription factors and chromatin remodelers. Perturbation experiments combined with single-cell RNA sequencing (scRNA-seq) highlighted the critical role of ZEB2 in both mouse and human somitogenesis.
This study provides a rich resource for the developmental and synthetic biology communities, offering new insights into mammalian embryogenesis.